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Gel Permeation Chromatography/Size Exclusion Chromatography (GPC/SEC) columns are critical for separating molecules based on their hydrodynamic volume. The performance and accuracy of your analysis directly depend on selecting the appropriate column. Investing in a high-quality GPC-SEC column ensures reliable results for polymer characterization and molecular weight determination.
Convenient packaging: 4 pieces per pack for easy storage and management for continued use.
C18 Column | Single Column Chromatography | High Quality Materials: Made with sturdy PP tubing and C18 filter to ensure durability and reliable performance.
Versatile compatibility: Compatible with HPLC systems and suitable for a variety of chromatography applications.
Economical Reuse: Designed to be reused five times, providing cost-effective use and sustainability.
Convenient packaging: 4 pieces per pack for easy storage and management for continued use.
C18 Column | Single Column Chromatography | High Quality Materials: Made with sturdy PP tubing and C18 filter to ensure durability and reliable performance.
Versatile compatibility: Compatible with HPLC systems and suitable for a variety of chromatography applications.
Economical Reuse: Designed to be reused five times, providing cost-effective use and sustainability.
Robust Material Composition: engineered from G3.3 high borosilicate Glass for longevity, enhanced chemical resistance and reliability; equipped with a PTFE valve for added durability and chemical protection
Precision Sand Plate Design: features a G2 sand plate at the base, providing consistent and accurate separation with sand core apertures ranging from 40 to 80 Micrometers, ensuring fine control over separation processes
Seamless Integration: designed with a 19 mm inner diameter and 8 inch length, complete with a standard 24/40 interface at the top for easy connection to laboratory equipment, ensuring compatibility and smooth workflow
Versatile Laboratory Tool: tailored for the precise separation of mixtures containing diverse organic compounds, facilitating comprehensive experimentation and research in various laboratory settings
Application: Suitable for high-capacity preparative chromatography and an extra-long effective length for demanding separations. Select the stationary phase, packing height, eluent, and flow method according to the sample and validated separation...
Integrated Reservoir: The one-piece 1000mL reservoir provides solvent head capacity without a separate reservoir-to-column joint, simplifying assembly and reducing the number of potential connection points.
Joint, Flow Control, and Packing Support: The 24/40 Outer Joint accepts matching 24/40 Inner Joint components. The 2mm PTFE stopcock regulates eluent flow. This open-bore model has no built-in frit; install a suitable support above the outlet, keep...
Recommended Assembly: Compatible accessory options include an AST13 PTFE joint sleeve, HMC0207 solvent reservoir, HMCCSS metal joint clip, and HMC0201-series flow control adapter. Verify the matching 24/40 joint size, connection type, and intended...
SEPARATION || Chromatography column designed for general chromatography analysis - Used in the separation of mixtures between the stationary and mobile phase
COST-EFFECTIVE || For education and preparation - A cost-effective technique that works with a vast array of substances
STOPCOCK || Includes a rotaflow stopcock for ease of use and a 19/26 socket
SINTERED DISC || Features a sintered disc with 0 porosity - Ideal for coarse filtration and gas dispersion
High-quality material: Medical-grade PP polypropylene cylinder, highly biocompatible, resistant to chemical corrosion, suitable for various experimental reagents.
Low adsorption: Hydrophilic precision sieve plate, non-specific binding is low, reduces protein loss, ensures accurate experimental data.
Flexible and versatile: Standard Rüll interface, can be connected in series with equipment, supports self-filling of packing materials, suitable for protein purification and sample separation.
Complete specifications: Available in 50-piece and 100-piece packaging options, customizable packaging specifications are available to meet the needs of small-scale experiments and batch testing.
Last update on 2026-10-17 / Affiliate links / Images from Amazon Product Advertising API
How to Choose the Best Lab Chromatography GPC-SEC Columns
Column Chemistry
The stationary phase chemistry is paramount. Common options include polystyrene-divinylbenzene (PS-DVB), silica, and various functionalized polymers. PS-DVB is versatile for organic solvents and a wide range of polymers. Silica-based columns are often used for aqueous GPC/SEC or when specific interactions are desired.
Particle Size and Pore Size
Particle size impacts efficiency and back pressure. Smaller particles generally offer higher resolution but also increase back pressure, requiring more robust pumping systems. Pore size determines the molecular weight range the column can effectively separate. Ensure the pore size distribution of the column aligns with the molecular weight range of the samples you intend to analyze.
Solvent Compatibility
Verify that the column's stationary phase and packing material are compatible with the mobile phase (solvent) you plan to use. Incompatible solvents can degrade the column, leading to poor performance and reduced lifespan. Always consult the manufacturer's specifications for recommended solvents.
Frequently Asked Questions
What is the primary function of a GPC-SEC column?
A GPC-SEC column separates molecules based on their size in solution. It works by allowing larger molecules to elute first, while smaller molecules take a longer path through the pores and elute later.
How do I choose the right pore size for my application?
The pore size should be chosen to encompass the molecular weight range of your sample. If analyzing very large polymers, you'll need columns with larger pores, whereas smaller molecules require smaller pores for effective separation.
Can I use any solvent with a GPC-SEC column?
No, solvent compatibility is crucial. The stationary phase and packing material must be stable in the chosen mobile phase. Using an incompatible solvent can damage the column and compromise your results.
What is the difference between GPC and SEC?
While often used interchangeably, GPC (Gel Permeation Chromatography) typically refers to separation in organic solvents, while SEC (Size Exclusion Chromatography) is the broader term that includes separation in aqueous mobile phases as well.
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