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Gel Permeation Chromatography/Size Exclusion Chromatography (GPC/SEC) columns are critical for separating molecules based on their hydrodynamic volume. The performance and accuracy of your analysis directly depend on selecting the appropriate column. Investing in a high-quality GPC-SEC column ensures reliable results for polymer characterization and molecular weight determination.
SEPARATION || Chromatography column designed for general chromatography analysis - Used in the separation of mixtures between the stationary and mobile phase
COST-EFFECTIVE || For education and preparation - A cost-effective technique that works with a vast array of substances
STOPCOCK || Includes a rotaflow stopcock for ease of use and a 19/26 socket
SINTERED DISC || Features a sintered disc with 0 porosity - Ideal for coarse filtration and gas dispersion
DURABLE & STURDY || Made of high quality Borosilicate 3.3 glass - Built to last and withstand multiple uses
High-capacity chromatography column with 1000 mL reservoir for large-scale flash and preparative chromatography on a 24/40 joint system.
60 mm outer diameter and approximately 457 mm effective bed length provide an exceptionally large packing volume for demanding separations.
Integrated 1000 mL reservoir facilitates solvent and sample loading for extended runs and high sample loads.
PTFE stopcock at the bottom enables smooth, accurate control of solvent flow; wetted surfaces are PTFE and borosilicate glass for broad solvent compatibility.
Designed for gravity or low-pressure flash chromatography under modest positive pressure; clamp the column securely and operate in a fume hood behind a safety shield. Stand, clamps and chromatography media are not included.
Premium Construction: made from G3.3 high borosilicate glass, ensuring durability and resistance to chemical corrosion, with a polytetrafluoroethylene (PTFE) valve for added reliability
Enhanced Separation: features a G2 sand plate at the bottom with a sand core pore size ranging from 40 to 80 micrometers, facilitating efficient separation of mixed samples containing various organic compounds
Precise Dimensions: with an inner diameter of 13mm and an effective length of 203mm, equipped with a standard 24/40 joint at the top for seamless integration into laboratory setups
Versatile Application: ideal for separating mixed samples containing multiple organic compounds, making it a valuable tool for various laboratory experiments and analyses
Satisfaction Guarantee: in case of any damage upon receipt, please contact us immediately for prompt assistance and resolution; your satisfaction is our priority
Long fritted chromatography column for higher-capacity flash and preparative chromatography.
26 mm outer diameter and approximately 457 mm effective length with frit provide a deep bed volume for demanding separations.
Fritted glass disc supports the packed bed and prevents loss of adsorbent while maintaining solvent flow.
PTFE stopcock allows smooth, precise regulation of solvent flow under gravity or low-pressure conditions.
Borosilicate 3.3 hand-blown glass with uniform wall thickness offers clarity, chemical resistance and thermal shock durability; column must be clamped securely and used behind a safety shield in a fume hood.
Last update on 2026-07-18 / Affiliate links / Images from Amazon Product Advertising API
How to Choose the Best Lab Chromatography GPC-SEC Columns
Column Chemistry
The stationary phase chemistry is paramount. Common options include polystyrene-divinylbenzene (PS-DVB), silica, and various functionalized polymers. PS-DVB is versatile for organic solvents and a wide range of polymers. Silica-based columns are often used for aqueous GPC/SEC or when specific interactions are desired.
Particle Size and Pore Size
Particle size impacts efficiency and back pressure. Smaller particles generally offer higher resolution but also increase back pressure, requiring more robust pumping systems. Pore size determines the molecular weight range the column can effectively separate. Ensure the pore size distribution of the column aligns with the molecular weight range of the samples you intend to analyze.
Solvent Compatibility
Verify that the column's stationary phase and packing material are compatible with the mobile phase (solvent) you plan to use. Incompatible solvents can degrade the column, leading to poor performance and reduced lifespan. Always consult the manufacturer's specifications for recommended solvents.
Frequently Asked Questions
What is the primary function of a GPC-SEC column?
A GPC-SEC column separates molecules based on their size in solution. It works by allowing larger molecules to elute first, while smaller molecules take a longer path through the pores and elute later.
How do I choose the right pore size for my application?
The pore size should be chosen to encompass the molecular weight range of your sample. If analyzing very large polymers, you'll need columns with larger pores, whereas smaller molecules require smaller pores for effective separation.
Can I use any solvent with a GPC-SEC column?
No, solvent compatibility is crucial. The stationary phase and packing material must be stable in the chosen mobile phase. Using an incompatible solvent can damage the column and compromise your results.
What is the difference between GPC and SEC?
While often used interchangeably, GPC (Gel Permeation Chromatography) typically refers to separation in organic solvents, while SEC (Size Exclusion Chromatography) is the broader term that includes separation in aqueous mobile phases as well.
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