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Thin Layer Chromatography (TLC) plates are essential tools for separating and analyzing chemical compounds. The performance and reliability of your results depend heavily on selecting the appropriate TLC plates for your specific application. Understanding key features will ensure efficient and accurate experimental outcomes in various laboratory settings.
Classical Silica Gel GF254 Plate: With calcined gypsum and fluorescent indicator, 60 Å pore size, it produces sharp dark spots under 254 nm UV light for stain-free visualization, trace analysis, and easy identification of colorless substances
Glass-Backed TLC Plate: The silica gel (SiO₂·nH₂O) layer is inert to most acids and bases, except strong alkalis and HF. The rigid glass backing provides outstanding resistance to solvents
High-Resolution Separation: This TLC plate delivers high theoretical plate numbers, superior separation performance, compact, round spots free of tailing, enabling clear detection of trace components
Short Separation Time: This lab thin layer chromatography TLC plates feature short development time, high color development sensitivity, large separation capacity, and significantly reduces experimental duration
High Separation Performance: Silica gel TLC plates provide clear and consistent separation with well-defined spots, helping improve accuracy when analyzing mixed samples.
Fast & Clear Development: Designed for quick development, these thin layer chromatography plates produce sharp, low-diffusion spots for easy observation and comparison.
Glass Backed Stability: Glass backed TLC plate offers a flat and rigid surface with good solvent resistance. Suitable for use with a wide range of common laboratory solvents.
GF254 Fluorescent Indicator: Built-in fluorescent indicator allows compounds to be visualized under 254 nm UV light, making it easier to locate colorless samples without additional staining.
【Reliable Silica Gel Coating】The plates feature a high-quality silica gel layer, designed to deliver consistent separation results. This coating ensures good adsorption properties, allowing for clear and distinct spot formation, which is essential for...
【Optimal Size & Sturdy Construction】Each plate measures 25mm x 75mm, a practical size that fits well with common lab setups and procedures. The glass backing offers enhanced stability during handling and development, reducing the risk of bending or...
【Ample Quantity for Multiple Uses】This package includes 80 thin layer chromatography plates, providing a sufficient supply for ongoing lab work, classroom experiments, or research projects. Whether you're conducting routine analyses or teaching...
【Versatile for Various Applications】Perfectly suited for school science classes, university laboratories, and research facilities. These TLC plates can be used for a range of tasks, including identifying components in mixtures, monitoring reaction...
High-Sensitivity F254 Fluorescent Indicator Detect UV-active and non-chromophoric compounds instantly under 254nm UV light without chemical derivatization. 250μm uniform silica gel 60 layer delivers sharp, consistent separation bands for reliable...
Durable Aluminum Backing for Crack-Free Handling Flexible 0.1mm aluminum substrate resists curling, delamination and breakage in humid lab environments. Sturdy backing allows easy cutting, marking and spotting; compatible with all common solvent systems...
Lab-Grade Consistency Meets USP Standards Each batch verified for uniform layer thickness, particle size and activity. Complies with USP chromatography guidelines; suitable for pharmaceutical impurity profiling, natural product isolation and food...
Multiple Sizes for Every Application Available in 20×20cm (10/box), 10×10cm (20/box) and 2.5×7.5cm (80/box) pre-cut formats. Ideal for analytical TLC, 2D-TLC, method development, educational teaching labs and small-scale preparative separations
High-Resolution Separation: Our TLC plate achieves superior separation with high theoretical plate numbers and compact sample spots, enabling precise analysis of complex mixtures
Rapid Results: The silica gel plates provide sharp, non-diffused spots after short development times for clear detection of trace components
Glass Backed TLC Plate: SiO₂·nH₂O layer inert to most acids or bases (excluding strong alkalis and HF). Rigid glass backing ensures excellent solvent resistance
F254 Fluorescent Indicator: Delivers sharp, dark compound spots under 254nm light, enabling stain-free visualization for trace analysis and effortless colorless sample separation
Material: High-efficiency the thin-layer chromatography silica gel plate is made of high-purity thin-layer chromatography silica gel (powder) mixed with a certain amount of binder. The surface of the plate is pure white, flat and even, fine, and no...
Model: GF254, It contains both calcined gypsum and fluorescent agent. Diameter of 60 Å，It can be used to observe fluorescence under ultraviolet light with a wavelength of 254, Plate Format is 2.5 x 7.5 cm
Good Separation Effect: The spots are small and convenient for series analysis. The separation time is short, and more than 5 groups can be separated per minute
High Sensitivity: The spots are clear, no spreading and no trailing. Large separation capacity. It’s suitable for thin layer scanning quantitative analysis
【Easily Cut】 ：Silica gel GF254， It contains both calcined gypsum and fluorescent agent. Diameter of 60 Å，It can be used to observe fluorescence under ultraviolet light with a wavelength of 254. The aluminum back can be easily cut open with...
【Good separation effect】: The spots are small and convenient for series analysis. The separation time is short, and more than 5 groups can be separated per minute.
【High sensitivity】: The spots are clear, no spreading and no trailing. Large separation capacity. It’s suitable for thin layer scanning quantitative analysis.
【CUSTOMER SERVICE】:If you encounter any problem, please contact us. we will help you solve the problem as soon as possible.
Set of 10: glass-backed TLC silica gel plates offering outstanding consistency; primarily utilized for qualitative and quantitative analysis of lab specimens; well-suited for applications with modest sample quantities
Size: 7.9 x 7.9 Inch, pack of 10L; layer thickness: 1000 µm; particle size: 10 - 40 µm; PH: 6.2 - 6.8; pore size: 60 Å
Silica Precision: crafted using unaltered silica gel and a gypsum binder to ensure superior separation accuracy, heightened sensitivity, and a diverse range of chromogenic agent options
Fluorescence Visibility: the inclusion of a fluorescence indicator enables the observation of fluorescence under UV light at a wavelength of 254
High-Sensitivity F254 Fluorescent Indicator Detect UV-active and non-chromophoric compounds instantly under 254nm UV light without chemical derivatization. 250μm uniform silica gel 60 layer delivers sharp, consistent separation bands for reliable...
Durable Aluminum Backing for Crack-Free Handling Flexible 0.1mm aluminum substrate resists curling, delamination and breakage in humid lab environments. Sturdy backing allows easy cutting, marking and spotting; compatible with all common solvent systems...
Lab-Grade Consistency Meets USP Standards Each batch verified for uniform layer thickness, particle size and activity. Complies with USP chromatography guidelines; suitable for pharmaceutical impurity profiling, natural product isolation and food...
Multiple Sizes for Every Application Available in 20×20cm (10/box), 10×10cm (20/box) and 2.5×7.5cm (80/box) pre-cut formats. Ideal for analytical TLC, 2D-TLC, method development, educational teaching labs and small-scale preparative separations
Last update on 2026-10-15 / Affiliate links / Images from Amazon Product Advertising API
How to Choose the Best Lab Thin Layer Chromatography TLC Plates
Key Considerations for TLC Plates
When selecting lab TLC plates, focus on these critical factors to ensure optimal performance for your analytical needs:
Stationary Phase Type: The most common stationary phase is silica gel, which is versatile for a wide range of compounds. Alumina is another option, often preferred for separating basic compounds or those sensitive to acidic conditions. Consider the polarity of the compounds you intend to separate; silica gel is polar, while alumina is less polar. Some plates also offer specific modifications, such as C18, for reversed-phase chromatography.
Binder and Fluorescent Indicator: Most plates use a binder to hold the stationary phase to the plate, providing mechanical strength. A fluorescent indicator (usually UV 254) is embedded in the layer to help visualize colorless compounds under UV light. Ensure the indicator's wavelength (e.g., 254 nm) matches your UV visualization equipment.
Plate Size and Material: Plates come in various standard sizes (e.g., 5x20 cm, 20x20 cm) to suit different chamber types and sample loads. The plate material can be glass, aluminum foil, or plastic. Glass plates offer rigidity and reusability but are fragile. Aluminum and plastic plates are more flexible and less prone to breakage, making them suitable for field applications or when cost is a primary concern.
Frequently Asked Questions
What is the difference between silica gel and alumina TLC plates?
Silica gel is a polar stationary phase widely used for separating moderately polar to non-polar compounds. Alumina is less polar than silica gel and is often preferred for separating basic compounds or those sensitive to acidic conditions.
Why is a fluorescent indicator important on TLC plates?
A fluorescent indicator, typically activated at 254 nm, is embedded in the stationary phase. It allows for the visualization of colorless compounds by quenching the fluorescence where the compounds are located under UV light.
Can TLC plates be reused?
Generally, TLC plates are designed for single use, especially after sample separation and visualization. While glass-backed plates can be cleaned, it's often difficult to remove all residual compounds and may affect subsequent separation quality.
What are the advantages of plastic or aluminum backed TLC plates?
Plastic and aluminum backed plates are more flexible and significantly less prone to breakage compared to glass-backed plates. This makes them ideal for applications where durability is important or for cost-sensitive laboratory work.
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