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Thin Layer Chromatography (TLC) plates are essential tools for separating and analyzing chemical compounds. The performance and reliability of your results depend heavily on selecting the appropriate TLC plates for your specific application. Understanding key features will ensure efficient and accurate experimental outcomes in various laboratory settings.
High Separation Performance: Silica gel TLC plates provide clear and consistent separation with well-defined spots, helping improve accuracy when analyzing mixed samples.
Fast & Clear Development: Designed for quick development, these thin layer chromatography plates produce sharp, low-diffusion spots for easy observation and comparison.
Glass Backed Stability: Glass backed TLC plate offers a flat and rigid surface with good solvent resistance. Suitable for use with a wide range of common laboratory solvents.
GF254 Fluorescent Indicator: Built-in fluorescent indicator allows compounds to be visualized under 254 nm UV light, making it easier to locate colorless samples without additional staining.
Standard Size & Versatile Use: 25 × 75 mm TLC plates, pack of 80. Suitable for general laboratory use, including educational settings, research experiments, and routine sample separation.
【Reliable Silica Gel Coating】The plates feature a high-quality silica gel layer, designed to deliver consistent separation results. This coating ensures good adsorption properties, allowing for clear and distinct spot formation, which is essential for...
【Optimal Size & Sturdy Construction】Each plate measures 25mm x 75mm, a practical size that fits well with common lab setups and procedures. The glass backing offers enhanced stability during handling and development, reducing the risk of bending or...
【Ample Quantity for Multiple Uses】This package includes 80 thin layer chromatography plates, providing a sufficient supply for ongoing lab work, classroom experiments, or research projects. Whether you're conducting routine analyses or teaching...
【Versatile for Various Applications】Perfectly suited for school science classes, university laboratories, and research facilities. These TLC plates can be used for a range of tasks, including identifying components in mixtures, monitoring reaction...
【Convenient to Work With】Ready to use right out of the package, eliminating the need for time-consuming preparation. The standard size and consistent quality make them easy to integrate into existing lab protocols, helping to streamline experiments...
Classical Silica Gel GF254 Plate: With calcined gypsum and fluorescent indicator, 60 Å pore size, it produces sharp dark spots under 254 nm UV light for stain-free visualization, trace analysis, and easy identification of colorless substances
Glass-Backed TLC Plate: The silica gel (SiO₂·nH₂O) layer is inert to most acids and bases, except strong alkalis and HF. The rigid glass backing provides outstanding resistance to solvents
High-Resolution Separation: This TLC plate delivers high theoretical plate numbers, superior separation performance, compact, round spots free of tailing, enabling clear detection of trace components
Short Separation Time: This lab thin layer chromatography TLC plates feature short development time, high color development sensitivity, large separation capacity, and significantly reduces experimental duration
Wide Application: Standard 25×75 mm TLC plates (80 per package, 1.76 lbs) deliver excellent performance for quantitative analysis, including pharmaceutical quality control, food testing, environmental analysis, cosmetics, and scientific research...
High Sensitivity with F254 Fluorescent Indicator Detect compounds instantly under UV 254nm light without chemical developers—ideal for analyzing non-chromophoric substances like lipids and terpenes
Large 2.5*7.5inch） Size for Complex Separations Maximize sample capacity for 2D-TLC, preparative chromatography, or multi-sample screening in pharmaceutical and biochemistry labs
Durable Aluminum Backing & Uniform Thickness 250μm silica gel layer on sturdy aluminum substrate ensures crack-free handling and consistent separation performance in humid conditions
Ready-to-Use & Pre-Cut for Convenience Each plate pre-scored into 20x20cm (total 10 plates/box); sealed in moisture-proof packaging to preserve activity
Trusted by Research Labs Worldwide Compatible with all common solvents and staining methods (iodine, sulfuric acid, etc.)
High-Resolution Separation: Our TLC plate achieves superior separation with high theoretical plate numbers and compact sample spots, enabling precise analysis of complex mixtures
Rapid Results: The silica gel plates provide sharp, non-diffused spots after short development times for clear detection of trace components
Glass Backed TLC Plate: SiO₂·nH₂O layer inert to most acids or bases (excluding strong alkalis and HF). Rigid glass backing ensures excellent solvent resistance
F254 Fluorescent Indicator: Delivers sharp, dark compound spots under 254nm light, enabling stain-free visualization for trace analysis and effortless colorless sample separation
Broad Application: Standard 25x75mm TLC plates (80/pkg, 0.8kg) ideal for pharmaceutical quality control, food testing, environmental analysis, and research labs
Material: High-efficiency the thin-layer chromatography silica gel plate is made of high-purity thin-layer chromatography silica gel (powder) mixed with a certain amount of binder. The surface of the plate is pure white, flat and even, fine, and no...
Model: GF254, It contains both calcined gypsum and fluorescent agent. Diameter of 60 Å，It can be used to observe fluorescence under ultraviolet light with a wavelength of 254, Plate Format is 2.5 x 7.5 cm
Good Separation Effect: The spots are small and convenient for series analysis. The separation time is short, and more than 5 groups can be separated per minute
High Sensitivity: The spots are clear, no spreading and no trailing. Large separation capacity. It’s suitable for thin layer scanning quantitative analysis
Application: Biochemical analysis, food industry,cosmetic industry,separating multicomponent pharmaceutical formulations, also can be used to identify natural products like essential oils or volatile oil, fixed oil, glycosides, waxes, alkaloids, etc
Compact Size for Multiple Sample Screening Ideal for teaching labs and quick tests, these 2.5 x 7.5 cm TLC plates save space and reduce material waste while enabling efficient multi-sample analysis.
High Sensitivity with F254 Fluorescent Indicator Detect compounds instantly under UV 254nm light without chemical developers—perfect for analyzing lipids, terpenes, and non-chromophoric substances.
Durable Aluminum Backing & Uniform Silica Layer 250μm silica gel on sturdy aluminum ensures crack-free handling and consistent separation performance, even in humid lab conditions.
Ready-to-Use & Pre-Cut for Convenience Each plate is pre-scored and sealed in moisture-proof packaging with desiccant—80 plates per box for extended use.
Trusted by Educators & Research Labs Compatible with all common solvents and staining methods (iodine, sulfuric acid), ideal for biochemistry, pharmaceutical, and teaching labs.
【Easily Cut】 ：Silica gel GF254， It contains both calcined gypsum and fluorescent agent. Diameter of 60 Å，It can be used to observe fluorescence under ultraviolet light with a wavelength of 254. The aluminum back can be easily cut open with...
【Good separation effect】: The spots are small and convenient for series analysis. The separation time is short, and more than 5 groups can be separated per minute.
【High sensitivity】: The spots are clear, no spreading and no trailing. Large separation capacity. It’s suitable for thin layer scanning quantitative analysis.
【CUSTOMER SERVICE】:If you encounter any problem, please contact us. we will help you solve the problem as soon as possible.
【Glass Backed TLC Classical Silica Gel Plate】 ：Silica gel GF254， It contains both calcined gypsum and fluorescent agent. Diameter of 60 Å，It can be used to observe fluorescence under ultraviolet light with a wavelength of 254.
【Good separation effect】: The spots are small and convenient for series analysis. The separation time is short, and more than 5 groups can be separated per minute.
【High sensitivity】: The spots are clear, no spreading and no trailing. Large separation capacity. It’s suitable for thin layer scanning quantitative analysis.
【EXCELLENT CUSTOMER SERVICE】We are dedicated in providing the best products and services to customers. If you encounter any problem, please don’t hesitate to contact us. We will reply your e-mail in no more than 8 hours since we receive it, and we...
Product Name：TLC Plates Aluminum Backed Silicone Gel
About the product: Silicone GF254, contains calcined gypsum and fluorescent agent, can show color, aluminum backing can be easily cut with scissors, size 10 cm x 10 cm
Product features: 1. Good separation effect 2. Small spot, easy to analyze in series 3. Short separation time 4. High sensitivity, clear spot, no diffusion, no trailing 5. Constant Rf value
Applicable fields: Suitable for quantitative analysis of thin layer scanning, widely used in precision instruments, medicine, petrochemical, food, leather, home appliances and other fields
Last update on 2026-07-17 / Affiliate links / Images from Amazon Product Advertising API
How to Choose the Best Lab Thin Layer Chromatography TLC Plates
Key Considerations for TLC Plates
When selecting lab TLC plates, focus on these critical factors to ensure optimal performance for your analytical needs:
Stationary Phase Type: The most common stationary phase is silica gel, which is versatile for a wide range of compounds. Alumina is another option, often preferred for separating basic compounds or those sensitive to acidic conditions. Consider the polarity of the compounds you intend to separate; silica gel is polar, while alumina is less polar. Some plates also offer specific modifications, such as C18, for reversed-phase chromatography.
Binder and Fluorescent Indicator: Most plates use a binder to hold the stationary phase to the plate, providing mechanical strength. A fluorescent indicator (usually UV 254) is embedded in the layer to help visualize colorless compounds under UV light. Ensure the indicator's wavelength (e.g., 254 nm) matches your UV visualization equipment.
Plate Size and Material: Plates come in various standard sizes (e.g., 5x20 cm, 20x20 cm) to suit different chamber types and sample loads. The plate material can be glass, aluminum foil, or plastic. Glass plates offer rigidity and reusability but are fragile. Aluminum and plastic plates are more flexible and less prone to breakage, making them suitable for field applications or when cost is a primary concern.
Frequently Asked Questions
What is the difference between silica gel and alumina TLC plates?
Silica gel is a polar stationary phase widely used for separating moderately polar to non-polar compounds. Alumina is less polar than silica gel and is often preferred for separating basic compounds or those sensitive to acidic conditions.
Why is a fluorescent indicator important on TLC plates?
A fluorescent indicator, typically activated at 254 nm, is embedded in the stationary phase. It allows for the visualization of colorless compounds by quenching the fluorescence where the compounds are located under UV light.
Can TLC plates be reused?
Generally, TLC plates are designed for single use, especially after sample separation and visualization. While glass-backed plates can be cleaned, it's often difficult to remove all residual compounds and may affect subsequent separation quality.
What are the advantages of plastic or aluminum backed TLC plates?
Plastic and aluminum backed plates are more flexible and significantly less prone to breakage compared to glass-backed plates. This makes them ideal for applications where durability is important or for cost-sensitive laboratory work.
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