Hydrophilic Interaction Liquid Chromatography (HILIC) columns are essential tools for separating polar and hydrophilic compounds that are poorly retained in reversed-phase chromatography. Selecting the appropriate HILIC column is crucial for achieving optimal separation efficiency, peak shape, and reproducibility in your analytical workflows.
Looking for the best Lab Chromatography LC HILIC Columns?
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- Tops tooled for a No. 4 rubber stopper
- Sealed-in coarse porosity fritted disc (40 to 60 μm)
- Outflow tubes are larger in diameter to elimainte the mearing of the definition bands of effluent caused by the tube being filled and the solvent mixed before being expelled
- Outflow tubes are long enough to protrude through a rubber stopper
- Chromatography column
- 24/40 Joint; G2 40~80 μm Nominal Max. pore size
- With Fritted Disc and PTFE Stopcock
- 25mm in column O.D. And 203mm in effective length
- StonyLab ensures premium products and premium customer service; if received damaged, please contact us immediately; we will solve it until satisfied
- PEEK column threads offer outstanding durability
- Precision borosilicate glass columns provide inertness, visibility, and performance
- Superior frit placement adds bed support and even distribution of sample flow
- Reusable columns let you pack your own media specific to your application
- PEEK column threads offer outstanding durability
- Precision borosilicate glass columns provide inertness, visibility, and performance
- Superior frit placement adds bed support and even distribution of sample flow
- Reusable columns let you pack your own media specific to your application
- Glass chromatography column with sintered disc, ideal for in-column operations in chemical experiments
- 30mm column O.D. and 300 mm effective length
- Standard 24/40 taper outer joint at the top, equipped with a fritted disc and a PTFE stopcock
- Made of high borosilicate glass, featuring a thick-walled design for reusability; maximum withstand temperature 250°C
- Warranty: Labasics 1-year limited warranty; if received damaged, please contact us immediately; we will solve it until satisfied
- Bundle includes: 1 x glass chromatography column, 1 x 500 mL glass chromatography reservoir
- The glass chromatography column is made of borosilicate glass with a 24/40 standard joint, PTFE stopcock, and fritted disc, effective column length 11.8 inches, column O.D. 1.97 inches
- The 500 mL chromatography reservoir is corrosion resistant, transparent for easy observation, facilitating fluid storage and control
- This bundle is suitable for separation experiments, research, and educational applications involving liquid handling and chromatography
- Warranty: 1-year coverage period for your peace of mind, if received damaged, please let us know and we will solve it promptly
- 40mm column O.D. and 300mm effective length
- 24/40 standard taper top outer joint
- With fritted disc and PTFE stopcock
- G3 15 - 40 μm Nominal Max. Pore Size
- Made by hand-blowing to ensure achieving the best performance
- High-Precision Chromatography Column: features a 1 inch inner diameter and 8 inch effective length, equipped with a 24/40 standard taper upper joint for secure connections
- G2 Sand Plug at Base: includes a G2 sand plug with a sand core aperture of 0.0016-0.0031 inch at the column's base, ensuring efficient separation of complex mixtures
- Durable Borosilicate Glass Construction: made from G3.3 borosilicate glass, known for its high thermal resistance and chemical inertness, ensuring durability and reliability in laboratory settings
- PTFE Valve: features a polytetrafluoroethylene (PTFE) valve for precise control over flow and separation processes, suitable for separating mixed samples containing various organic compounds
- Warranty: stonylab 1-year limited warranty; if received damaged, please contact us immediately; we will solve it until satisfied
- Chromatography Adapter
- Flow-control adapter
- 24/40 Joint
- Borosilicate glass; heavy duty design; reusable and premium; maximum withstand temperature 200°C
- StonyLab ensures premium products and premium customer service; if received damaged, please contact us immediately; we will solve it until satisfied
- PEEK column threads offer outstanding durability
- Precision borosilicate glass columns provide inertness, visibility, and performance
- Superior frit placement adds bed support and even distribution of sample flow
- Reusable columns let you pack your own media specific to your application
- Each glass column assembly is supplied preassembled with 25 μm PE frits and comes with all the tubing, fittings, and connections you need to get started
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Last update on 2026-03-19 / Affiliate links / Images from Amazon Product Advertising API
How to Choose the Best Lab Chromatography LC HILIC Columns
Key Factors for Choosing a HILIC Column
When selecting a lab chromatography HILIC column, consider the following:- Column Chemistry and Stationary Phase: The choice of stationary phase significantly impacts retention and selectivity. Common options include bare silica, amide, cyano, diol, and zwitterionic phases. Bare silica offers strong retention but can be prone to peak tailing. Amide and diol phases provide a good balance of retention and reproducibility, while zwitterionic phases offer unique selectivity for certain compound classes. Consider the polarity of your analytes and the desired separation mechanism when making your selection.
- Particle Size and Pore Size: Particle size affects column efficiency and backpressure. Smaller particles (e.g., 3 µm or 1.7 µm) generally provide higher resolution but increase backpressure, requiring compatible instrumentation. Larger particles (e.g., 5 µm) are more forgiving with less demanding systems. Pore size influences analyte diffusion and access to the stationary phase surface, impacting retention and separation of larger molecules.
- Column Dimensions: Standard analytical columns (e.g., 150-250 mm length, 4.6 mm ID) are versatile for method development. Shorter columns (e.g., 50-100 mm) offer faster analysis times, while narrower ID columns (e.g., 2.1 mm) reduce solvent consumption. Ensure the column dimensions are compatible with your instrument's flow rate capabilities and sample injection volume.