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Hydrophilic Interaction Liquid Chromatography (HILIC) columns are essential tools for separating polar and hydrophilic compounds that are poorly retained in reversed-phase chromatography. Selecting the appropriate HILIC column is crucial for achieving optimal separation efficiency, peak shape, and reproducibility in your analytical workflows.
Sealed-in coarse porosity fritted disc (40 to 60 μm)
Outflow tubes are larger in diameter to elimainte the mearing of the definition bands of effluent caused by the tube being filled and the solvent mixed before being expelled
Outflow tubes are long enough to protrude through a rubber stopper
Robust Material Composition: engineered from G3.3 high borosilicate Glass for longevity, enhanced chemical resistance and reliability; equipped with a PTFE valve for added durability and chemical protection
Precision Sand Plate Design: features a G2 sand plate at the base, providing consistent and accurate separation with sand core apertures ranging from 40 to 80 Micrometers, ensuring fine control over separation processes
Seamless Integration: designed with a 19 mm inner diameter and 8 inch length, complete with a standard 24/40 interface at the top for easy connection to laboratory equipment, ensuring compatibility and smooth workflow
Versatile Laboratory Tool: tailored for the precise separation of mixtures containing diverse organic compounds, facilitating comprehensive experimentation and research in various laboratory settings
Warranty: if received damaged, please contact us immediately; we will solve it until satisfied
Precision borosilicate glass columns provide inertness, visibility, and performance
Superior frit placement adds bed support and even distribution of sample flow
Reusable columns let you pack your own media specific to your application
Each glass column assembly is supplied preassembled with 25 μm PE frits and comes with all the tubing, fittings, and connections you need to get started
Premium Construction: made from G3.3 high borosilicate glass, ensuring durability and resistance to chemical corrosion, with a polytetrafluoroethylene (PTFE) valve for added reliability
Enhanced Separation: features a G2 sand plate at the bottom with a sand core pore size ranging from 40 to 80 micrometers, facilitating efficient separation of mixed samples containing various organic compounds
Precise Dimensions: with an inner diameter of 13mm and an effective length of 203mm, equipped with a standard 24/40 joint at the top for seamless integration into laboratory setups
Versatile Application: ideal for separating mixed samples containing multiple organic compounds, making it a valuable tool for various laboratory experiments and analyses
Satisfaction Guarantee: in case of any damage upon receipt, please contact us immediately for prompt assistance and resolution; your satisfaction is our priority
Convenient Packaging:Comes in packs of 4, making it easy to store and manage for consistent use.
C18 Chromatigraphic Column|Spe Column Chromatography|High-Quality Material:Crafted with a robust PP tube and C18 filter, ensuring durability and reliable performance.
Versatile Compatibility:Compatible with HPLC systems, ideal for various chromatography applications.
Economical Reuse:Designed for 5 regenerations, offering cost-effective use and sustainability.
Efficient Pretreatment:Optimized for hydrophobic compound removal, with a pH range of 2~8.
Last update on 2026-09-03 / Affiliate links / Images from Amazon Product Advertising API
How to Choose the Best Lab Chromatography LC HILIC Columns
Key Factors for Choosing a HILIC Column
When selecting a lab chromatography HILIC column, consider the following:
Column Chemistry and Stationary Phase: The choice of stationary phase significantly impacts retention and selectivity. Common options include bare silica, amide, cyano, diol, and zwitterionic phases. Bare silica offers strong retention but can be prone to peak tailing. Amide and diol phases provide a good balance of retention and reproducibility, while zwitterionic phases offer unique selectivity for certain compound classes. Consider the polarity of your analytes and the desired separation mechanism when making your selection.
Particle Size and Pore Size: Particle size affects column efficiency and backpressure. Smaller particles (e.g., 3 µm or 1.7 µm) generally provide higher resolution but increase backpressure, requiring compatible instrumentation. Larger particles (e.g., 5 µm) are more forgiving with less demanding systems. Pore size influences analyte diffusion and access to the stationary phase surface, impacting retention and separation of larger molecules.
Column Dimensions: Standard analytical columns (e.g., 150-250 mm length, 4.6 mm ID) are versatile for method development. Shorter columns (e.g., 50-100 mm) offer faster analysis times, while narrower ID columns (e.g., 2.1 mm) reduce solvent consumption. Ensure the column dimensions are compatible with your instrument's flow rate capabilities and sample injection volume.
Frequently Asked Questions
What are the main differences between HILIC and reversed-phase chromatography?
Reversed-phase chromatography uses a non-polar stationary phase and a polar mobile phase, retaining non-polar analytes longer. HILIC uses a polar stationary phase and a less polar (often high organic content) mobile phase, retaining polar analytes through partitioning into an adsorbed water layer. This makes HILIC ideal for compounds poorly retained in reversed-phase.
Which HILIC stationary phase is best for polar analytes?
For highly polar analytes, amide, diol, or zwitterionic stationary phases are often preferred. These phases offer strong hydrophilic interactions and can provide good retention and selectivity for a wide range of polar compounds. Bare silica can also be effective but may require careful mobile phase optimization to manage peak shape.
How does mobile phase composition affect HILIC separations?
The mobile phase in HILIC typically consists of a high percentage of organic solvent (e.g., acetonitrile) and a smaller percentage of aqueous buffer. The water content is critical, as it forms the adsorbed water layer on the stationary phase where analytes partition. Adjusting the organic content and buffer concentration can significantly alter retention and selectivity.
Can HILIC columns be used with standard HPLC systems?
Yes, most HILIC columns are designed for use with standard HPLC and UHPLC systems. However, if you are using columns with very small particles (e.g., sub-2 u00b5m), ensure your instrument is rated for the higher backpressures generated. Always check the manufacturer's specifications for compatibility.
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